Two men have been charged in connection with the felling of the Sycamore Gap tree.
Daniel Graham, 38, and Adam Carruthers, 31, have been charged with causing criminal damage after the tree was cut down last September.
They have both also been charged with causing criminal damage to Hadrian's Wall.
There was a national outcry in September when the much-loved, 200-year-old Northumberland tree, which stood in a dramatic dip on Hadrian's Wall, was found to have been cut down.
Teams from the National Trust collected material at the scene from which they hoped could be "viable for propagating" - or to produce new saplings and trees directly from the Sycamore Gap.
Officials said they are also "hoping" the trunk of the original tree could still regrow - but it could be three years before they know whether this is a possibility.
They are due to appear at Newcastle Magistrates' Court on 15 May.
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